Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.235784 |
Chromosome: | chromosome_11 |
Location: | 1540352 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467753 | HMO2 | Heme oxygenase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCCGGAGCTGAGTCCCAGTGGCGCGCAACA |
Internal bar code: | GGGACGAAGCAGGCAGGAAGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 824 |
LEAP-Seq percent confirming: | 99.3735 |
LEAP-Seq n confirming: | 1269 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGTCGTGTGACGCTAGAA |
Suggested primer 2: | TTACGGGTTCTCCATCCTTG |