Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.235798 |
Chromosome: | chromosome_10 |
Location: | 2319642 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g434800 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCGGTGTTCTGCCGCTTGGGACGCACAAG |
Internal bar code: | CGGTCGATGGAGCAGTCAGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 671 |
LEAP-Seq percent confirming: | 96.957 |
LEAP-Seq n confirming: | 5321 |
LEAP-Seq n nonconfirming: | 167 |
LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCACGGTCAACTACCTCAT |
Suggested primer 2: | GTAGTGACCGGGACAAGGAA |