| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235808 |
| Chromosome: | chromosome_9 |
| Location: | 3324647 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g388393 | ELG13 | Exostosin-like glycosyltransferase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGAGAAGCCGTCGAACGAGAACAGCACG |
| Internal bar code: | CTCTTACGGGGAATCATTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 793 |
| LEAP-Seq percent confirming: | 99.352 |
| LEAP-Seq n confirming: | 11652 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAACCGAGTGAGTGAGT |
| Suggested primer 2: | TTCAACGATGCAAAGCACTC |