| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235808 |
| Chromosome: | chromosome_9 |
| Location: | 3324649 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g388393 | ELG13 | Exostosin-like glycosyltransferase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGACATGGCATACAGCGGTGGCGTGCGTC |
| Internal bar code: | GGGTTCGGCTGGGTCGGAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 76.0061 |
| LEAP-Seq n confirming: | 5005 |
| LEAP-Seq n nonconfirming: | 1580 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAACCGAGTGAGTGAGT |
| Suggested primer 2: | TTCAACGATGCAAAGCACTC |