Insertion junction: LMJ.RY0402.235814_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.235814
Chromosome:chromosome 12
Location:725055
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g495100 PSR1 (1 of 1) PF00249//PF14379 - Myb-like DNA-binding domain (Myb_DNA-binding) // MYB-CC type transfactor, LHEQLE motif (Myb_CC_LHEQLE); Phosphorus starvation response 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAACTTGCTCGCGCTCCCTTGGCGGAGCT
Internal bar code:GGGTGCCCGAAGGCGAAGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:673
LEAP-Seq percent confirming:99.2135
LEAP-Seq n confirming:883
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCCCGTTTGCATAAAGTC
Suggested primer 2:CCTGTCCCATCACACACTTG