| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235822 |
| Chromosome: | chromosome 2 |
| Location: | 348174 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075600 | (1 of 1) K10908 - DNA-directed RNA polymerase, mitochondrial [EC:2.7.7.6] (POLRMT, RPO41) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCGATGTGCGCTCAAGTACCGCCTGGA |
| Internal bar code: | TCCGGGAGACGTGTATCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 10.4313 |
| LEAP-Seq n confirming: | 578 |
| LEAP-Seq n nonconfirming: | 4963 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAAGAATGCACAAGGGAA |
| Suggested primer 2: | CACAACAACAACGTCCAAGG |