| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235850 |
| Chromosome: | chromosome 10 |
| Location: | 5159462 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456554 | CSC5 | Chlamydomonadale-specific family C protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACACGGGCCAAGCCTGGCGGAGGCACC |
| Internal bar code: | TGTGCGCGGCGGGATATCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 99.2559 |
| LEAP-Seq n confirming: | 1734 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTAGGCTGTGCAGGTTTC |
| Suggested primer 2: | ACTGGGATGGCAAACGTAAG |