| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235861 |
| Chromosome: | chromosome 17 |
| Location: | 3350681 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723500 | (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAGCACTGGGCACTTGCAACGGCGGCAG |
| Internal bar code: | ATAGGTCACTATGGGACTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.2826 |
| LEAP-Seq n confirming: | 692 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCTGGCTTTGTTTGAA |
| Suggested primer 2: | AGATGGAGTACGACAACGGG |