| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235872 |
| Chromosome: | chromosome 16 |
| Location: | 5122621 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683900 | GDPD2,GDP2 | (1 of 3) K01126 - glycerophosphoryl diester phosphodiesterase (E3.1.4.46, glpQ, ugpQ); Glycerophosphoryl diester phosphodiesterase family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGGTCGCGCATGCGCAAGCATTTGCA |
| Internal bar code: | CCTTACATGCCCTGTGTTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 98.7133 |
| LEAP-Seq n confirming: | 9590 |
| LEAP-Seq n nonconfirming: | 125 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTTCAAAATCGTGGGTT |
| Suggested primer 2: | ATGCCCACCAAGACCTACAG |