Insertion junction: LMJ.RY0402.235888_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.235888
Chromosome:chromosome 6
Location:4091696
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278219 SSA6 cilia-sensing, structure and/or assembly; (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCAGTGCATTGAGATCGCAGTTAAATA
Internal bar code:GTTGTCTCAATGCCCGTAGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:561
LEAP-Seq percent confirming:98.6406
LEAP-Seq n confirming:1161
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCTGTTGTGCGAAAAATC
Suggested primer 2:AGTTGGCCAAAGGACAGATG