Insertion junction: LMJ.RY0402.235888_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.235888
Chromosome:chromosome 6
Location:4091731
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g278219 SSA6 cilia-sensing, structure and/or assembly; (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTAGAACGCTCTCATGAGGTCCGATGGC
Internal bar code:ACCTCACAAGCTTCTGGCGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:112
LEAP-Seq percent confirming:98.913
LEAP-Seq n confirming:455
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GAATGGATTGGAGGAACGTG
Suggested primer 2:TTGCTGTTGTGCGAAAAATC