| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235889 |
| Chromosome: | chromosome 14 |
| Location: | 3844570 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632759 | (1 of 2) 4.3.1.19 - Threonine ammonia-lyase / Threonine dehydratase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGCCGGTGGCGGCGGCGGCTGCGGCTC |
| Internal bar code: | GGGCTGTCAGTGTGTTTGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 96.3415 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAACAGTTGGGGGAGAGT |
| Suggested primer 2: | CGCAACGTTACCTCCTCCTA |