| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235903 |
| Chromosome: | chromosome 1 |
| Location: | 2747769 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016250 | MLO3,MLO4 | (1 of 5) PF03094 - Mlo family (Mlo); MLO family protein | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTCATTCGGGCACACGTGGTAGCAGCT |
| Internal bar code: | TGGATCAAGTGATGTTCGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.5825 |
| LEAP-Seq n confirming: | 7872 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCCCACTGTAAGCCGTTG |
| Suggested primer 2: | TCCAAACGTAGGTTTCAGGC |