| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235911 |
| Chromosome: | chromosome 10 |
| Location: | 5208243 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456950 | CEP10 | Cysteine endopeptidase; (1 of 1) IPR000668//IPR008979//IPR013128//IPR018392//IPR029062 - Peptidase C1A, papain C-terminal // Galactose-binding domain-like // Peptidase C1A // LysM domain // Class I glutamine amidotransferase-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGATCTTGGGCGGAGGCGGAGCCGTC |
| Internal bar code: | CCCCTGGGGATCTGGGCTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 98.8076 |
| LEAP-Seq n confirming: | 2486 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGGAGCATTCAAGTCAG |
| Suggested primer 2: | CGGTCAGTCGTCCTTTTCAT |