| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235915 |
| Chromosome: | chromosome 1 |
| Location: | 5552016 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039450 | (1 of 2) PTHR34548//PTHR34548:SF2 - FAMILY NOT NAMED // PROTEIN TIC 21, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGCGCGTGGTTGGTCCGGTGGTGTGTGC |
| Internal bar code: | GTTGCAAGGGACGTTTAAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 98.6076 |
| LEAP-Seq n confirming: | 1558 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGGCAAATTTACGTTCT |
| Suggested primer 2: | CAGCCGTAGTCTCGGAAGTC |