| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235917 |
| Chromosome: | chromosome 15 |
| Location: | 537439 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g637100 | (1 of 1) K04523 - ubiquilin (UBQLN, DSK2); DSK2 homolog | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGCTGTATCAAAATTCCAACACGCGCT |
| Internal bar code: | GGTCACCTGCGCTTGCCCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 98.3213 |
| LEAP-Seq n confirming: | 9547 |
| LEAP-Seq n nonconfirming: | 163 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCAAATACAGGTGGGTT |
| Suggested primer 2: | AACGGAACATCTAACGCACC |