| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235941 |
| Chromosome: | chromosome 10 |
| Location: | 4537375 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGAGACAACTGCCCCCGTACCACCCTC |
| Internal bar code: | TTACGTTTGCATGGTAAGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 81.3559 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACCTGACAAGCCCCAAT |
| Suggested primer 2: | GAGATGAAAACAGCGCACAA |