Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.235953 |
Chromosome: | chromosome_13 |
Location: | 352789 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g564400 | FAP161 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTAACAAACTCCTGGTAATGACAAGCGTCA |
Internal bar code: | AGGCTTTCCTGTGCCGGTGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 363 |
LEAP-Seq percent confirming: | 98.5061 |
LEAP-Seq n confirming: | 1055 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTGAGATACGCCATGCCTC |
Suggested primer 2: | GGAGAACAACCAGTCCCTGA |