| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235965 |
| Chromosome: | chromosome 17 |
| Location: | 87939 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGTTGGGGCAGGTAGGCGAGGGAGTTG |
| Internal bar code: | GCCGTATCAGGCGCTTCGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 98.8111 |
| LEAP-Seq n confirming: | 1496 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTATAGGGCCGCTGTGTT |
| Suggested primer 2: | TTAGCATTTCGACTTCGGCT |