Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.235990 |
Chromosome: | chromosome 9 |
Location: | 2675690 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g389850 | (1 of 29) IPR013320 - Concanavalin A-like lectin/glucanase domain | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGCGGCTACGAAGCCCGAGGCCCATGT |
Internal bar code: | GTTGGAAATCGGGTTTGTACCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 184 |
LEAP-Seq percent confirming: | 95.5882 |
LEAP-Seq n confirming: | 65 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAATTCAAGCGAGGCGTCTT |
Suggested primer 2: | ATGCGTCGGTCCGTTACTAC |