| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236006 |
| Chromosome: | chromosome 2 |
| Location: | 5690957 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110000 | (1 of 1) PF07974//PF13920 - EGF-like domain (EGF_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCAGCAATGACCAGACACAGATGCGCA |
| Internal bar code: | GCGCCTCGTGGAGTGCGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 98.9929 |
| LEAP-Seq n confirming: | 4030 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACATGTTGGTCAGGGCTC |
| Suggested primer 2: | GCGTATGAGTGTGTGATGGG |