Insertion junction: LMJ.RY0402.236006_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.236006
Chromosome:chromosome 12
Location:1746563
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g512550 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAGTGCGGCACTAACGCGGGCGGTTACA
Internal bar code:TCGCGTTGGGTATATTGGTTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:196
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACGCGAAGACGTTGTTCAG
Suggested primer 2:CATTCGTGTACAGCGACCAC