| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236007 |
| Chromosome: | chromosome 17 |
| Location: | 4039685 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729050 | (1 of 1) PTHR13309:SF0 - NUCLEAR FRAGILE X MENTAL RETARDATION-INTERACTING PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTTAATGAACAGCGCTCGCCGTGGCATG |
| Internal bar code: | TATCTGGCTATTTTTGGACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 98.857 |
| LEAP-Seq n confirming: | 3373 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGATGACGACTTTTTGCTG |
| Suggested primer 2: | GCATTTTGGCCATGTTTCTT |