Insertion junction: LMJ.RY0402.236010_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.236010
Chromosome:chromosome 6
Location:7215365
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g297904 (1 of 1) K10863 - aprataxin [EC:3.-.-.-] (APTX) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGCTGAAGAGCCATATTGTGGCCTGCG
Internal bar code:GGCTCAAAGTGACCACTGCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1108
LEAP-Seq percent confirming:99.0352
LEAP-Seq n confirming:4619
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCAACCTACCCGTGATGC
Suggested primer 2:ACACAATGTGACCCCATCCT