| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236015 |
| Chromosome: | chromosome 10 |
| Location: | 3809101 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446950 | (1 of 2) PTHR23181//PTHR23181:SF7 - RHO GTPASE ACTIVATING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGCGCCTTCTTAGCCGGTGGCCGTTCG |
| Internal bar code: | TAGGCGCCCCCGTCGAAACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1147 |
| LEAP-Seq percent confirming: | 99.5455 |
| LEAP-Seq n confirming: | 26063 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTGGATTTCTCTCTGCC |
| Suggested primer 2: | CGCACGCACCTTTAATTTTT |