| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236033 |
| Chromosome: | chromosome 16 |
| Location: | 2017891 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656900 | (1 of 77) IPR001623 - DnaJ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAAACTCTGCTCGTGCAAGCTAGAATAG |
| Internal bar code: | GCACTTTCAAGCCAATCGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 98.3486 |
| LEAP-Seq n confirming: | 536 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGGAGCGTAATCGACAG |
| Suggested primer 2: | GGCCTATGAACACACGGTTT |