| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236055 |
| Chromosome: | chromosome 12 |
| Location: | 5944758 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534400 | (1 of 18) IPR011051 - RmlC-like cupin domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGCAAAATAGCACGCAACGATGTTGT |
| Internal bar code: | AGCACCGGGATTTTACCGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 81.3852 |
| LEAP-Seq n confirming: | 3913 |
| LEAP-Seq n nonconfirming: | 895 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCAGGAACAGGAGCAAG |
| Suggested primer 2: | CCTGTGCCAGTTCCCAGTAT |