| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236068 |
| Chromosome: | chromosome 7 |
| Location: | 4537094 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343700 | OGD2 | Dihydrolipoamide succinyltransferase, oxoglutarate dehydrogenase E2 component; (1 of 2) K00658 - 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase) (DLST, sucB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAAGCTTCTGGGCCTATGGGTTATGAC |
| Internal bar code: | GTCATGGGGTGTGTCTAGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 99.4048 |
| LEAP-Seq n confirming: | 9687 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAATGCACAGTGTGGTTG |
| Suggested primer 2: | TTGTCTCACAGCAGTTTGCC |