| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236091 |
| Chromosome: | chromosome 12 |
| Location: | 3432025 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496500 | PHC70 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGATTGATTGCGGCGGCTGGTGCGAGCA |
| Internal bar code: | ACCTTGGATCTTGCGTACTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1123 |
| LEAP-Seq percent confirming: | 99.5877 |
| LEAP-Seq n confirming: | 22465 |
| LEAP-Seq n nonconfirming: | 93 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTACTGGATGAAGGGTGC |
| Suggested primer 2: | AGTCTCCGTTGGTTGGTTTG |