| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236111 |
| Chromosome: | chromosome 3 |
| Location: | 6048847 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191300 | LCI34,LCI35 | Low-CO2-inducible protein 35 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGCGACTGCGCACGCGCCCGCCACCGC |
| Internal bar code: | TGGAGTTGCCAACCGAGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 96.9262 |
| LEAP-Seq n confirming: | 946 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTAACACAAACACGGAA |
| Suggested primer 2: | TTCGTCATCTAGGCCGTCTT |