| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236112 |
| Chromosome: | chromosome 3 |
| Location: | 2966283 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTCCCGGCCCAATCCACGGCCTCCGAC |
| Internal bar code: | GCCGATAGTCTCTAGCAGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 98.6306 |
| LEAP-Seq n confirming: | 2881 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATCCCATGGGTACATGA |
| Suggested primer 2: | AAAAGAATCATGTACGGCGG |