Insertion junction: LMJ.RY0402.236112_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.236112
Chromosome:chromosome 16
Location:583920
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g692300 (1 of 6) 3.6.3.43 - Peptide-transporting ATPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCATGTTATGCCACGCCACAAACGCCGCC
Internal bar code:GGACTCCCCTCGTAACCATACG

Confirmation - LEAP-Seq

LEAP-Seq distance:206
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTACCGGTAGCAGTATCGGC
Suggested primer 2:CGTGGTCTTTTGCCTGGTAT