| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236121 |
| Chromosome: | chromosome 9 |
| Location: | 1551980 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGCCCCAACTACGGTAGTTCTGCCTCC |
| Internal bar code: | CCGGGTAGGCTCCGGCGGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 97.733 |
| LEAP-Seq n confirming: | 6208 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAATTTGTTGCCGGTCGT |
| Suggested primer 2: | GTGACAGGGTACAGGCAGGT |