| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236139 |
| Chromosome: | chromosome 3 |
| Location: | 4097103 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172900 | (1 of 2) PF11371 - Protein of unknown function (DUF3172) (DUF3172) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTCTCTTCTCTCCAACGGAAAGTGGACC |
| Internal bar code: | TTTTCGGGATTTGTGATCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 98.6066 |
| LEAP-Seq n confirming: | 1203 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGCGCTAACAGGCTATG |
| Suggested primer 2: | CGGGCTGTTCTGCTAGTACC |