| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236199 |
| Chromosome: | chromosome 3 |
| Location: | 7704474 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203650 | (1 of 1) IPR009072//IPR013785 - Histone-fold // Aldolase-type TIM barrel | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCTCGCCTGACCAGAACGTGCCACAAG |
| Internal bar code: | GCGCGATGTAATTATACGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 52.518 |
| LEAP-Seq n confirming: | 146 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACGAGGTGGAGGATGTG |
| Suggested primer 2: | ACACCCAGCTATACATCCGC |