| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236229 |
| Chromosome: | chromosome 3 |
| Location: | 4515383 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176400 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCAGATGCGCCAGGAATGTAGACCAGG |
| Internal bar code: | CGGATGCGTGGCTCACTGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 99.6403 |
| LEAP-Seq n confirming: | 10803 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTACAGAGAAGTGGGGG |
| Suggested primer 2: | GAGGAATTGGTAAACGGGGT |