Insertion junction: LMJ.RY0402.236234_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.236234
Chromosome:chromosome 2
Location:4281902
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g099055 (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGAGTTGGTTAGCGTCAGTTGGAACAG
Internal bar code:GATCGTCATATTACTGTCTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:619
LEAP-Seq percent confirming:80.1986
LEAP-Seq n confirming:1373
LEAP-Seq n nonconfirming:339
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GAATGCCAGGTCCTGATGAT
Suggested primer 2:CAACGTCTCATGCTGCTCAT