| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236237 |
| Chromosome: | chromosome 17 |
| Location: | 3159535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721750 | ELG9 | Exostosin-like glycosyltransferase 9; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCAAACCAGCCCACAGTAGCGCCTCCC |
| Internal bar code: | TGGAGGTAGACCGTCGCCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 222 |
| LEAP-Seq percent confirming: | 99.4691 |
| LEAP-Seq n confirming: | 11428 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGTTGAATAGCACGCCA |
| Suggested primer 2: | CGGACTAGGCACGAGAGAAC |