| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236242 |
| Chromosome: | chromosome 7 |
| Location: | 5962794 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354500 | HSF2 | (1 of 2) K09419 - heat shock transcription factor, other eukaryote (HSFF); Heat shock transcription factor 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTATAGAATCACCCGATTGACGCGTTGC |
| Internal bar code: | TTCCAGGAAATGGGATGTATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 201 |
| LEAP-Seq percent confirming: | 98.1982 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACATCTCTTGTCCCAGGT |
| Suggested primer 2: | CGATTGTGCTGCTGGAAGTA |