Insertion junction: LMJ.RY0402.236244_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.236244
Chromosome:chromosome 17
Location:2631805
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g717200 (1 of 1) PTHR10060:SF24 - DEOXYRIBONUCLEASE TATDN3-RELATED intron|outside_mRNA
Cre17.g717250 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAAACAACACTCGCACAGCTTCTCTCA
Internal bar code:CGGGACGCTTGCGACGGTGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:692
LEAP-Seq percent confirming:92.5035
LEAP-Seq n confirming:654
LEAP-Seq n nonconfirming:53
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:AGATGCAGCCACCTCTCACT
Suggested primer 2:ATACGCGCATAAACGTCCTC