| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236244 |
| Chromosome: | chromosome 17 |
| Location: | 2631805 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g717200 | (1 of 1) PTHR10060:SF24 - DEOXYRIBONUCLEASE TATDN3-RELATED | intron|outside_mRNA | |
| Cre17.g717250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCGAGTACTCCGTATGTGCATGTTGGT |
| Internal bar code: | TAATTGAATCGACGGTCGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 97.3125 |
| LEAP-Seq n confirming: | 4490 |
| LEAP-Seq n nonconfirming: | 124 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGCAGCCACCTCTCACT |
| Suggested primer 2: | ATACGCGCATAAACGTCCTC |