| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236302 |
| Chromosome: | chromosome 11 |
| Location: | 1851068 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467950 | FAP383 | Flagellar Associated Protein 383 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCACGTGCCCGCCGCCCCTCGCATGTA |
| Internal bar code: | GCGTAGAATTAGAGGAGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 99.1617 |
| LEAP-Seq n confirming: | 1656 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAAGGTCAAGGTGGAGA |
| Suggested primer 2: | CAGAGGAAGCATGGAGGAAG |