| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236302 |
| Chromosome: | chromosome 11 |
| Location: | 1851072 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467950 | FAP383 | Flagellar Associated Protein 383 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACCCCCCGGACCCCCCTGATCAAGCGG |
| Internal bar code: | CCTTTGTATGCGGGCTCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.3037 |
| LEAP-Seq n confirming: | 7559 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAAGGTCAAGGTGGAGA |
| Suggested primer 2: | GCAGCGATCAACATGAGAGA |