| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236309 |
| Chromosome: | chromosome 2 |
| Location: | 4342806 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099300 | ANK21 | (1 of 15) PF13857 - Ankyrin repeats (many copies) (Ank_5); Predicted protein with ankyrin repeats | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTCACGCGACCTCCTCCTTCATGTCCC |
| Internal bar code: | TTGTTGGTTATCTGGATTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 87.2888 |
| LEAP-Seq n confirming: | 8886 |
| LEAP-Seq n nonconfirming: | 1294 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGGTGTGATACCATCAG |
| Suggested primer 2: | GGATCAAGACGGATCAAGGA |