Insertion junction: LMJ.RY0402.236334_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.236334
Chromosome:chromosome 1
Location:1037450
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g006000 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGTAATCACTTTATCGGTTTCTTCATA
Internal bar code:TCGTGCAGCCAACTCAGCAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:520
LEAP-Seq percent confirming:92.7011
LEAP-Seq n confirming:3353
LEAP-Seq n nonconfirming:264
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACCACAGGTTCCAAAGC
Suggested primer 2:ACAAGGGGGAGTTGTGTCTG