Insertion junction: LMJ.RY0402.236341_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.236341
Chromosome:chromosome 10
Location:974378
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g424775 CTP2,PCH1,PAA1 Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATATAGAAACGGGCACAGGTCCCAATCCA
Internal bar code:CAATACGTAGAAACATTGTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:597
LEAP-Seq percent confirming:99.0789
LEAP-Seq n confirming:1506
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGTCGTGTCGCTCAAATA
Suggested primer 2:ATCGGATCGGTCAGAACTTG