| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236344 |
| Chromosome: | chromosome 4 |
| Location: | 3022007 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225050 | (1 of 3) PF16166 - Chloroplast import apparatus Tic20-like (TIC20) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCACTCACTCGCCAGCGTGCAACCACA |
| Internal bar code: | GCACGGCGACGGTGGCTGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 580 |
| LEAP-Seq percent confirming: | 98.8802 |
| LEAP-Seq n confirming: | 2649 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTCCTGGAGAAGACAGC |
| Suggested primer 2: | AATTTTGTAGCGTGTACCGGA |