Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.236350 |
Chromosome: | chromosome_6 |
Location: | 2796534 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g271150 | FAP74 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACCGCACGTGTGGAGCAGCGTGCAAGGGA |
Internal bar code: | GCGAATGATGATCAGCCGCTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 446 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 387 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGAGCTCACGCTAAACTTCC |
Suggested primer 2: | ATGGACTCCAACACGTCCTC |