| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236353 |
| Chromosome: | chromosome 16 |
| Location: | 1029067 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649250 | (1 of 4) PF13911 - AhpC/TSA antioxidant enzyme (AhpC-TSA_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCGGCTGTACGGCGGTCGGTGTGGGT |
| Internal bar code: | GATGTCCAGGTTTAGTTTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 99.2444 |
| LEAP-Seq n confirming: | 13528 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACCACAGCTTACAGGCCC |
| Suggested primer 2: | TGGTTGACCGTTGACGAGTA |