Insertion junction: LMJ.RY0402.236355_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.236355
Chromosome:chromosome 5
Location:1750475
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g242600 CGL106 (1 of 2) IPR000679//IPR013088 - Zinc finger, GATA-type // Zinc finger, NHR/GATA-type; zinc finger GATA transcription factor 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGGGCGCAAACCATACATGTGGCCTGCA
Internal bar code:CGGGCTGCGTTGCCGTCCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1021
LEAP-Seq percent confirming:96.36
LEAP-Seq n confirming:2409
LEAP-Seq n nonconfirming:91
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGGGATGGTGTGTATCA
Suggested primer 2:TTGTGTGCTGGGTGTTTGAT